目的 研究正弦交变电磁场(SEMFs)对体外培养大鼠成骨细胞增殖与分化的影响.方法 体外分离培养大鼠颅骨成骨细胞,传代后随机分为6组,分别用频率50 Hz、强度为3.6 mT SEMFs处理,处理时间分别为每天0.5、1.0、1.5、2.0、2.5h.结果 SEMFs处理后3～5d,细胞呈现特征样分布.SEMFs处理后9d,0.5h组碱性磷酸酶(ALP)活性显著增加,ALP组织化学染色和钙化结节面积结果与ALP活性一致.在SEMFs处理后48 h和96 h,骨保护素和胶原Ⅰ mRNA表达水平明显高于对照组,尤以0.5h组最明显.结论 50 Hz、3.6 mT SEMFs可抑制成骨细胞增殖,但能促进体外培养成骨细胞分化成熟.%Objective To investigated the effect of 50-Hz 3. 6-mT sinusoidal electromagnetic fields (SEMFs) on the proliferation and differentiation of osteoblasts in vitro. Methods The newborn rat calvarial osteoblasts were isolated by enzyme digestion and randomly divided into 6 groups after one passage. The treatment groups under 50-Hz 3. 6-mT SEMFs and controls without SEMFs treatment. The cells were exposed in the SEMFs for 0. 5 h, 1. 0 h, 1. 5 h, 2. 0 h, and 2. 5 h. They were observed under the contrast phase microscope each day. The calcified nodules were stained by alizarin red. The SEMFs were arranged in spiral appearance after 3 to 5 days. Results The SEMFs showed characteristic distribution 3 to 5 days after SEMFs treatment. On the 9lh day after treatment, the activity of alkaline phosphatase (ALP) significantly increased in the 0. 5-h group, whereas the ALP histochemical straining results and the area of calcified nodules were consistent with ALP activity. In the 48-h and 96-h groups, the genetic expression levels of osteoprotegerin and collagen- I were significantly higher than that in the control group; particularly, the mRNA expression increased in the 0. 5-h group. Conclusion The SEMFs at 50-Hz 3. 6-mT could suppress the proliferation of osteoblasts
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