Objective To investigate the effect of CUE domain-containing protein 2 (CUEDC2) on the sensitivity of ovarian cancer cellstocisplatin and its mechanisms. Methods CUEDC2 gene in SKOV3 cells were knocked down by siRNA, and then MTS assay and Trypan blue staining technique were used to detect the effect of cisplatin on the cell proliferation of si-control group's (treated with si-control 50 nmol/L) and Si-CUEDC2 group's (treated with Si-CUEDC2 50 nmol/L) SKOV3 cells. SKOV3 cells were grouped into drug group (cisplatin 8 μ mol/L), si-control group (cisplatin 8 μ mol/L, si-control 50 nmol/L), Si-CUEDC2 group (cisplatin 8 μ mol/L, Si-CUEDC2 50 nmol/L), non-treatment group, and the expressions of related protein in MAPK were evaluated by western blotting. Results There was no significant difference in the quantity of SKOV3 cells between Si-CUEDC2 without cisplatin treatment group and si-control group. Compared with si-control group, the quantity of SKOV3 cells decreased significantly in the Si-CUEDC2 with cisplatin treatment group (P ＜ 0.05). Under the effect of cisplatin, p38 phosphorylation level increased while p-ERK phosphorylation level decreased in SKOV3 cells with knockdown of CUEDC2. Conclusion Knocking down CUEDC2 can increase the sensitivity of SKOV3 cells to cisplatin, and CUEDC2 may play a role in regulating the effect of cisplatin on ovarian cancer cell proliferation through MAPK pathway.%目的 探讨CUEDC2对卵巢癌细胞顺铂敏感性的影响及相关作用机制.方法采用siRNA技术敲低浆液性卵巢癌(SKOV3)细胞株中CUEDC2基因,Western blotting法检测siRNA技术在细胞中的敲除效果,采用MTS比色法、锥虫蓝染色计数方法检测在顺铂作用下si-control组与Si-CUEDC2组SKOV3细胞增殖情况,Western blotting法检测加药组(顺铂8μmol/L)、si-control组(顺铂8μmol/L,si-control 50 nmol/L)、Si-CUEDC2组(顺铂8 μ mol/L,Si-CUEDC2 50 nmol/L)、空白组SKOV3细胞中促分裂素原活化蛋白激酶(MAPK)通路中相关蛋白的表达水平.结果未加顺铂处理的Si-CUEDC2组与si-control组SKOV3细胞数量无差异,而经顺铂处理的si-CUEDC2组与si-control组相比SKOV3细胞数明显减少(P ＜0.05);si-CUEDC2的SKOV3细胞在顺铂的作用下Phosphorylatio-p38高表达,Phosphorylatio-ERK低表达.结论敲低CUEDC2可显著增加卵巢癌细胞对顺铂的敏感性;CUEDC2可能通过MAPK通路影响顺铂对卵巢癌细胞增殖的抑制作用.
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