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【6h】Agrobacterium-mediated transformation of perennial ryegrass (Lolium perenne L.) for cold tolerance.

机译农杆菌介导的多年生黑麦草(黑麦草)的耐寒性转化。

【摘要】Perennial ryegrass (Lolium perenne L.) is an agronomically important grass grown in the temperate regions of the world. It is widely used as a forage crop and turfgrass for its high quality and yield. However, its growth is limited due to its inability to survive in extreme winter temperatures. The aim of the present study is to obtain cold tolerant perennial ryegrass by transforming it the cold regulated transcriptional activator Lp CBF3 gene using the Agrobacterium-mediated gene transfer method and evaluate overexpression of the LpCBF3 gene under the control of the constitutive promoter, cauliflower mosaic virus 35S (CaMV35S). Embryogenic calli of perennial ryegrass cv. 'Inspire' were transformed by an Agrobacterium strain EHA105 containing a modified pFGC5941 plasmid.;Results of the study showed that transgenic perennial ryegrass overexpressing the LpCBF3 gene was successfully regenerated. Presence and stable integration of the gene in the genome was confirmed by PCR analysis and Southern hybridization. Glufosinate resistance and electrolyte leakage tests indicated the expression of the gene in the transformants. Cold acclimated transgenic plants showed increased tolerance to freezing temperature. In this study, 35S:LpCBF3 transgenic plants did not show growth retardation but had reduced tillering ability.

【摘要机译】多年生黑麦草(Lolium perenne L.)是一种在世界上温带地区生长的重要农艺草。由于其高品质和高产量,它被广泛用作草料作物和草皮草。但是,由于无法在极端冬季温度下生存,其生长受到限制。本研究的目的是通过农杆菌介导的基因转移方法将冷调节的转录激活因子Lp CBF3基因转化为耐寒的多年生黑麦草,并在组成型启动子花椰菜花叶病毒的控制下评估LpCBF3基因的过表达。 35S(CaMV35S)。多年生黑麦草简历的胚性愈伤组织。用含有修饰的pFGC5941质粒的农杆菌菌株EHA105转化了'Inspire'。研究结果表明,成功表达了过表达LpCBF3基因的多年生黑麦草。通过PCR分析和Southern杂交证实了基因在基因组中的存在和稳定整合。草铵膦抗性和电解质渗漏测试表明该基因在转化体中的表达。冷适应的转基因植物显示出对冷冻温度的耐受性增加。在这项研究中,35S:LpCBF3转基因植物没有显示出生长迟缓,但分till能力降低。

【作者】Bales, Carmille Joanna C.;

【作者单位】Michigan State University.;

【年(卷),期】2010(),

【年度】2010

【页码】67 p.

【总页数】67

【原文格式】PDF

【正文语种】eng

【中图分类】;

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