首页> 外文学位 >Creation of a framework genetic linkage map of colonial bentgrass and the identification of genomic regions associated with dollar spot resistance.
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Creation of a framework genetic linkage map of colonial bentgrass and the identification of genomic regions associated with dollar spot resistance.

机译:殖民地宾草的框架遗传连锁图谱的创建以及与美元斑点抗性相关的基因组区域的鉴定。

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摘要

Creeping bentgrass (Agrostis stolonifera L. 2n=4x=28, A2A2A3A3) is a cool season turfgrass known for its lateral growth habit, fine texture and ability to tolerate low mowing heights. One of the biggest maintenance problems for creeping bentgrass is the fungal disease dollar spot caused by Sclerotinia homoeocarpa. Currently the disease is controlled through heavy fungicide use but a more practical long term approach lies through the development of resistant cultivars. Colonial bentgrass (A. capillaris L. 2n=4x=28, A1A1A2A2) is a species related to creeping bentgrass which is resistant to dollar spot. We have generated fertile creeping x colonial bentgrass interspecific hybrids. One plant, designated TH15, was backcrossed with a different creeping plant to generate a mapping population which segregated for dollar spot resistance. The objective of this thesis was to determine which areas of the colonial bentgrass genome are associated with dollar spot resistance. To do this we used the hybrid backcross population to generate a framework genetic linkage map of colonial bentgrass. We created and annotated EST libraries for both creeping and colonial bentgrass and used these libraries as a resource for mapping genes. A new approach to marker development, termed dideoxy polymorphism scanning, was developed to efficiently map genes on the colonial bentgrass map. The colonial bentgrass linkage map contains 212 AFLPs and 110 gene based markers and totals 1157 cM.;By comparing phenotypic data obtained from field trails we identified regions potentially associated with dollar spot resistance in colonial bentgrass. The segregation of resistance in the backcross population suggests a three gene recessive epistasis model for dollar spot resistance in colonial bentgrass. By comparing genotypes we identified colonial bentgrass loci on groups 2A1 and 3A1 that are common in all resistant individuals. We hypothesize that genes related to dollar spot resistance may lie on these groups. Other resistant backcross individuals not included in the mapping population also shared these loci proportions significantly higher then expected by random chance (p 0.05). This lends additional support to our model of dollar spot resistance in colonial bentgrass.
机译:爬行型草皮草(Agrostis stolonifera L. 2n = 4x = 28,A2A2A3A3)是凉爽的草皮草,以其侧向生长习性,良好的质地和耐低割草高度而闻名。蠕动草的最大维护问题之一是由果核盘菌引起的真菌病美元斑。目前,该病是通过大量使用杀真菌剂来控制的,但更长期的方法是通过开发抗性品种。殖民地草(A. capillaris L. 2n = 4x = 28,A1A1A2A2)是与蠕动草有关的物种,对美元斑有抵抗力。我们已经产生了可繁殖的蠕动x殖民地弯头草种间杂种。一株命名为TH15的植物与另一株爬行植物回交,以产生一个作图种群,这些种群按美元对点的抵抗力分离。本论文的目的是确定菌斑草基因组的哪些区域与美元斑点抗性相关。为此,我们使用杂种回交种群来生成殖民地原生草的框架遗传连锁图谱。我们为蠕虫和殖民地本草创建并注释了EST库,并将这些库用作定位基因的资源。开发了一种新的标记开发方法,称为双脱氧多态性扫描,可有效地在殖民地弯曲草地图上定位基因。殖民地草丛的连锁图谱包含212个AFLP和110个基于基因的标记,总计1157 cM。通过比较从田间试验获得的表型数据,我们确定了潜在的与殖民地草丛中美元斑点抗性相关的区域。回交种群中的抗药性分离表明,在殖民地弯曲草中美元斑点抗性的三个基因隐性上位性模型。通过比较基因型,我们在所有抗性个体中常见的2A1和3A1组上鉴定了结肠弯曲草基因座。我们假设与美元即期电阻相关的基因可能位于这些群体上。未包括在作图群体中的其他抗性回交个体也共享这些基因座比例,其显着高于随机机会所预期的比例(p <0.05)。这为我们在殖民地草丛中的美元现货阻力模型提供了额外的支持。

著录项

  • 作者

    Rotter, David.;

  • 作者单位

    Rutgers The State University of New Jersey - New Brunswick.;

  • 授予单位 Rutgers The State University of New Jersey - New Brunswick.;
  • 学科 Biology Molecular.;Agriculture Plant Pathology.;Agriculture Plant Culture.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 168 p.
  • 总页数 168
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;作物生物学原理、栽培技术与方法;植物病理学;
  • 关键词

  • 入库时间 2022-08-17 11:38:27

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