Development of desorption electrospray ionization mass spectrometry (DESI-MS) for applications in imaging and quantitation.

摘要

Further development of the ambient mass spectrometry ionization method desorption electrospray ionization (DESI) is presented with a focus on applications in imaging and quantitation. The second chapter is of a more fundamental nature and describes the DESI analysis of polar lipids. Three novel imaging applications are presented in the third chapter using DESI-MS. First, the chemical imaging of latent fingerprints is presented, in which both the shape and the chemical content of blotted fingerprints are determined. Second, imaging of lipids, including polar lipids, cholesterol, and cholesteryl esters, from atherosclerotic plaques is demonstrated. Finally, tissue imaging using a high mass-to-charge resolution and accuracy mass spectrometer is described. In the final chapter, various aspects of the use of DESI-MS for quantitation are explored. Basic method development is addressed in the first section, while the second extends the analysis to high throughput analysis.