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Suppression of Fusarium root and stem rot of greenhouse cucumber using composts containing fluorescent pseudomonads.

机译:使用含有荧光假单胞菌的堆肥抑制温室黄瓜的镰刀菌根和茎腐病。

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摘要

Three composts (Ball, dairy, and greenhouse) were tested for their ability to suppress development of fusarium root and stem rot of greenhouse cucumber, caused by Fusarium oxysporum f. sp. radicis-cucumeriunum (Forc). Dairy and greenhouse compost significantly reduced disease (P≤0.05), Ball compost had no effect. Microbial assessment of the composts showed a correlation between disease suppression and pseudomonad levels. Pseudomonas species were isolated using dilution plating. Strains showing the greatest in vitro antagonism were identified as P. aeruginosa. Growth room trials were conducted to test P. aeruginosa P23, the non-antagonistic P. maculicola and two strains of P. fluorescens for Forc suppression. Cucumbers grown in compost with P23 had reduced disease severity compared to controls. The locus for biosynthesis of the antibiotic 2,4-diacetylphloroglucinol (DAPG) was detected by Southern blot and confirmed by PCR; DAPG production by P. aeruginosa in liquid culture was detected by thin layer chromatography.;Keywords. Biological control, compost, Fusarium oxysporum f.sp. radicis-cucumerinum, Pseudomonas aeruginosa, antibiotics, 2,4-diacetylphloroglucinol.
机译:测试了三种堆肥(球,乳制品和温室)抑制由黄瓜枯萎病菌引起的温室黄瓜的镰刀菌根和茎腐病发展的能力。 sp。萝卜-黄瓜(Forc)。乳制品和温室堆肥可显着减少疾病(P≤0.05),球形堆肥无效果。堆肥的微生物评估显示,疾病抑制与假单胞菌水平之间存在相关性。使用稀释板分离假单胞菌种类。表现出最大的体外拮抗作用的菌株被鉴定为铜绿假单胞菌。进行了生长室试验,以测试铜绿假单胞菌P23,非拮抗性斑假单胞菌和两种菌株的荧光假单胞菌对Forc的抑制作用。与对照相比,在P23堆肥中生长的黄瓜降低了疾病的严重程度。通过Southern印迹检测并合成了抗生素2,4-二乙酰基间苯三酚(DAPG)的生物合成位点;薄层色谱法检测铜绿假单胞菌在液体培养物中DAPG的产生。生物防治,堆肥,尖镰孢f.sp.黄瓜,铜绿假单胞菌,抗生素,2,4-二乙酰基间苯三酚。

著录项

  • 作者

    Bradley, Geoffrey G.;

  • 作者单位

    Simon Fraser University (Canada).;

  • 授予单位 Simon Fraser University (Canada).;
  • 学科 Biology Microbiology.;Engineering Agricultural.;Agriculture Plant Pathology.
  • 学位 M.Sc.
  • 年度 2009
  • 页码 94 p.
  • 总页数 94
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 能源与动力工程;
  • 关键词

  • 入库时间 2022-08-17 11:38:08

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