Human kidney embryonic cells on synthetic diamond surfaces.

摘要

Diamond in thin film form, represents a promising material for tissue engineering scaffolds particularly due to its cytologically compatible properties. Here we study the effect of wettability and surface roughness on cellular adhesion. We use two types of diamond films, namely, terminated Ultrananocrystalline Diamond (UNCD) and Microcrystalline Diamond (MCD) surfaces. We modified the UNCD and MCD surfaces using: (a) UV light, and (b) piranha solution. We used contact angle measurements to confirm the altered wettability. The roughness of UNCD and MCD surfaces were measured by Atomic Force Microscopy (AFM). We used trypan blue assay to measure the cell viability of human embryonic kidney cells on modified diamond and control polystyrene culture dish surfaces. Finally, we established correlations between surface wettability, roughness, and cell adhesion force and survival on the aforementioned surfaces.