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Temporal comparisons on the genetic variation of the dusky gopher frog (Lithobates sevosus).

机译:灰鼠蛙(Lithobates sevosus)的遗传变异的时间比较。

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摘要

Monitoring temporal changes in genetic diversity within populations can provide vital information on future viability. The dusky gopher frog, Lithobates sevosus, exists in isolation with an estimated population size of 100--200 individuals, and previous research has shown that low genetic variability exists as a consequence of isolation and population size reduction. However, the temporal changes in genetic variation are not known. Therefore, my objectives were to (1) determine temporal trends in population genetic variation and implications for long-term viability of L. sevosus and (2) estimate effective population size. To accomplish these objectives, egg samples collected from 1997 to 2014 were genotyped for nine microsatellite loci. Observed and expected heterozygosity, allelic richness, and Wright's inbreeding coefficient were calculated for each year and differences between sample years were assessed. Additionally, overall and pair-wise FST values were calculated to test for temporal genetic structuring. To estimate effective population size, two single-sample estimators (Tallmon et al. 2008; Waples and Do 2008) and two temporal estimators (Waples 1989; Wang 2001) were used. The results show a stable, but low, level of genetic variation. Weak genetic structure (FST = 0.023 (95% CI 0.006-0.043)) was found among years, which can be attributed to the increased effects of genetic drift in small populations. L. sevosus currently has an estimated effective population size between 32.99-58.6 individuals. The ratios of effective size to census size per year are fairly large (∼0.5) and exhibit an increasing trend over time, which could possibly be explained by genetic compensation---or the lessening of genetic variation reduction in times with low population numbers. This research indicates the current management programs in place for L. sevosus have been effective at maintaining the genetic diversity present in the population; however, additional strategies need to be implemented to increase genetic diversity.
机译:监测种群内遗传多样性的时间变化可提供有关未来生存能力的重要信息。昏暗的地鼠蛙Lithobates sevosus孤立存在,估计种群数量为100--200个,先前的研究表明,由于孤立和种群数量减少,遗传变异性较低。但是,遗传变异的时间变化尚不清楚。因此,我的目标是(1)确定种群遗传变异的时间趋势以及对L. sevosus长期生存力的影响,以及(2)估算有效种群规模。为了实现这些目标,对1997年至2014年收集的卵样品进行了9个微卫星基因座的基因分型。每年计算观察到的和期望的杂合度,等位基因丰富度和赖特的近交系数,并评估样本年之间的差异。另外,计算总体和成对的FST值以测试时间遗传结构。为了估计有效人口规模,使用了两个单样本估计量(Tallmon等,2008; Waples和Do 2008)和两个时间估计量(Waples 1989; Wang 2001)。结果显示出稳定但低水平的遗传变异。几年间发现遗传结构较弱(FST = 0.023(95%CI 0.006-0.043)),这可以归因于小群体遗传漂移的影响增加。目前估计七叶乳杆菌的有效人口规模在32.99-58.6个人之间。每年的有效规模与人口普查规模之比相当大(〜0.5),并且随着时间的推移呈现出增加的趋势,这可以用遗传补偿来解释-或人口数量少时减少遗传变异的时间减少。这项研究表明,目前针对七叶猴的管理计划已经有效地维持了种群中的遗传多样性。但是,还需要执行其他策略来增加遗传多样性。

著录项

  • 作者

    Hinkson, Kristin Michelle.;

  • 作者单位

    Eastern Kentucky University.;

  • 授予单位 Eastern Kentucky University.;
  • 学科 Biology.;Genetics.
  • 学位 M.S.
  • 年度 2015
  • 页码 47 p.
  • 总页数 47
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 11:52:50

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