Kinetic studies of the hydrolysis of 3,5-diketo acids by fumarylacetoacetate fumarylhydrolase.

摘要

6,6-Dimethyl-3,5-dioxo-heptanoic acid, 3%,5-dioxo-hexanoic acid, 5-phenyl-3,5-dioxo-pentanoic acid and fumarylacetone were synthesized. The kinetics of the hydrolysis of these compounds by fumarylacetoacetate fumarylhydrolase were studied. The kinetic parameters of these compounds were compared with the kinetic parameters obtained for 2,4-diketo acids previously. The effect on activity, by fluoride, p-hydroxymecuribenzoate, cyanide, dihydroxytartaric acid, tartronic acid, oxalacetic acid, pyruvic acid and acetoacetic acid was also studied in order to further elucidate the mechanism of FAH.