THE USE OF OXYGEN CHIRAL PHOSPHODIESTERS IN STEREOCHEMICAL STUDIES OF ENZYMATIC NUCLEOTIDYL TRANSFER REACTIONS

摘要

The stereochemical course of the reaction catalyzed by the adenylate cyclase from Brevibacterium liquefaciens was investigated using the phosphorothioate substrate analog adenosine 5'-(0-1-thiotriphosphate) (ATP(alpha)S). The diastereomer of ATP(alpha)S having the S(,p) configuration at the (a.