The effects of crude oil on the growth of Chlamydomonas eugametos and Chlorella vulgaris.

摘要

Growth of Chlamydomonas eugametos and Chlorella vulgaris ;A new type of growth flask is described which permits bioassays to be done in the presence of whole crude oil. Experiments in these flasks showed that algal cells are not physically engulfed by oil and that oil toxicity to algae is primarily chemical in nature. Growth experiments in sterile oil revealed that strain ;Algal reactions to oil varied with the degree and diversity of microbial contamination of bioassay flasks, suggesting that the by-products of microbial respiration affect algal growth. Breakdown of oil occurred more rapidly and more extensively in mixed bacteria/algae cultures, suggesting that microbially-produced CO(,2) stimulates algal photosynthesis and that the oxygen produced during photosynthesis provides the O(,2) necessary for the bacterial breakdown of oil.;Growth of a heterotrophic (