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HEAVY METAL TOLERANCE AND METALLOTHIONEIN INDUCTION IN FATHEAD MINNOWS: RESULTS FROM FIELD AND LABORATORY INVESTIGATIONS.

机译:谷类小中的重金属耐受性和金属硫蛋白诱导:实地和实验室研究的结果。

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摘要

Toxicity tests were conducted to quantify the metal tolerance of two natural populations of fathead minnows (Pimephales promelas). One group of organisms was taken from a metal-contaminated flyash pond and the other population was collected from Newtown hatchery ponds. As determined in acute toxicity tests, ash pond minnows were significantly more tolerant to cadmium and copper than were hatchery minnows. At an exposure concentration of 6.0 mg Cd/l in moderately hard water, the median period of survival (LT(,50)) for flyash pond minnows was 50.0 hr compared to 6.8 hr for hatchery minnows. The LT(,50) values for ash pond and Newtown animals exposed to 0.50 mg Cu/l in moderately hard water were 17.0 and 4.5 hr, respectively. Both populations were about equally tolerant to zinc. After ash pond minnows were transferred to reconstituted water in the laboratory, tolerance to cadmium and copper decreased significantly after 7 days. Conversely, tolerance was increased in hatchery minnows following acclimation in the laboratory to sublethal concentrations of cadmium. For example, after exposing hatchery minnows for 35 days to 10 (mu)g Cd/l, acute toxicity tests gave LC(,50) values of 2.88 and 1.71 mg Cd/l for acclimated and control organisms, respectively. This induced tolerance in cadmium-acclimated hatchery minnows was lost when organisms were transferred to control water for 7 days.; Developing embryos of the fathead minnow and rainbow trout also acquired tolerance to cadmium. After eggs of the trout had been exposed to 0, 5, and 50 (mu)g Cd/l for 24 days, subsequent acute toxicity tests conducted on the newly hatched larvae gave 7-day LC(,50) values of 0.70, 1.59, and 2.02 (mu)g Cd/l, respectively.; The results support the conclusion that fathead minnows develop increased tolerance to cadmiium and copper following prolonged sublethal exposure to these metals, and that this metal-induced tolerance is not sustained once organisms are removed from toxicant stress. As determined in correlated biochemical studies, gill metallothionein concentrations correlated closely with the variations in tolerance exhibited during cadmium acclimation and deacclimation. Therefore, tolerance induction was attributed to increased production of metallothionein, a protein which selectively binds and sequesters cadmium, copper and certain other metals.
机译:进行了毒性测试,以量化两个自然种群的黑头min鱼(Pimephales promelas)的金属耐受性。一组生物是从一个金属污染的粉煤灰池中采集的,另一类是从纽敦孵化池中采集的。根据急性毒性试验确定,灰池鱼比孵化场min鱼对镉和铜的耐受性要强得多。在中度硬水中暴露浓度为6.0 mg Cd / l的情况下,粉煤灰塘min鱼的中位生存期(LT(,50))为50.0小时,而孵化场min鱼的中位生存期为6.8小时。在中度硬水中暴露于0.50 mg Cu / l的灰塘和Newtown动物的LT(,50)值分别为17.0和4.5 hr。两种种群对锌的耐受性大致相同。将灰pond鱼转移到实验室的再生水中后,对镉和铜的耐受性在7天后显着下降。相反,在实验室适应镉的致死浓度后,孵化场min鱼的耐受性增加。例如,将孵化场小鱼暴露于10μgCd / l下35天后,急性毒性试验得出,驯化生物和对照生物的LC(,50)值分别为2.88和1.71 mg Cd / l。当将有机物转移到控制水中7天后,这种对镉适应的孵化场min鱼的诱导耐受性就消失了。 head鱼和虹鳟鱼的发育中的胚胎也获得了对镉的耐受性。鳟鱼卵暴露于0、5和50μgCd / l 24天后,对新孵化的幼虫进行的急性毒性试验得出的7天LC(,50)值为0.70、1.59分别为2.02μgCd / l。该结果支持以下结论:黑头min鱼在长时间暴露于这些金属后,对镉和铜的耐受性增强,并且一旦将生物体从毒性胁迫中移出,这种金属诱导的耐受性就不会持续。如在相关的生化研究中所确定的,g金属硫蛋白的浓度与镉适应和去适应过程中表现出的耐受性变化密切相关。因此,耐受诱导归因于金属硫蛋白的增加,金属硫蛋白是一种选择性结合并螯合镉,铜和某些其他金属的蛋白质。

著录项

  • 作者

    BENSON, WILLIAM HAZLEHURST.;

  • 作者单位

    University of Kentucky.;

  • 授予单位 University of Kentucky.;
  • 学科 Biology General.; Agriculture Fisheries and Aquaculture.
  • 学位 Ph.D.
  • 年度 1984
  • 页码 137 p.
  • 总页数 137
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;水产、渔业;
  • 关键词

  • 入库时间 2022-08-17 11:51:09

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