Somaclonal variation in inbreds and testcrosses of maize (Zea mays L.) and the possible involvement of transposable elements.

摘要

The culture of plant cells in vitro results in the accumulation of genetic and cytogenetic modifications in both the cultured cells and plants regenerated from them which has been termed "somaclonal variation". The primary objectives of this research were to evaluate the amount of somaclonal variation in quantitatively inherited characters at both the inbred (R;At the hybrid level, FR27rhm R;Contrary to published experiments, all genotypes examined, Oh43, Al88, Al88 x Oh43, FR27rhm and 79-R4443 possess the 2.5 kb PvuII-Ac fragment but do not contain active Ac elements. A change in the genomic positions of Ac-homologous sequences, which should accompany Ac activation, was observed in only one of the 57 R;Using the inbred line FR27rhm, significant somaclonal variation was produced in R