Locations of genes controlling proanthocyanidin production in barley (Hordeum vulgare L.)

摘要

Cultivated barley (Hordeum vulgare L.) contains anthocyanins in the green organs of the plant and proanthocyanidins and catechins in the testa of the grain. Colloidal haze in beer is caused primarily by the complexing of malt proteins with malt and hop (Humulus lupulus L.) proanthocyanidins. Proanthocyanidin-free barley mutants have provided a means to stabilize beer, thus eliminating the need for special operations and avoiding the extra costs for additives used to eliminate haze. In these experiments, the chromosomal location and linkage relationships of mutant genes ant13 (Ant 582, and Ant 566) and ant17 (Ant 567) were evaluated by using male-sterile stocks and a multiple recessive marker stock, and the allelic relationships of ant genes of four proanthocyanidin-free mutant lines (Ant 566, Ant 582, Ant 597, and Ant 625) were tested.;Ant 566 and Ant 582 have mutant genes which are alleles at the same locus, which is ant13. Ant 597 and Ant 625 have mutant genes which are alleles at the same locus but which are different from ant13. Using male-sterile stocks and a multiple recessive marker stock, the ant13 gene showed association with the genes for male sterility, msg6, and orange lemma, o, which they are located on chromosome 6. A weighted average recombination value of p = 0.4563 $pm$ 0.1526 was obtained for msg6 and ant13. Also, a recombination value of p = 0.1824 $pm$ 0.0403 was obtained for o and ant13. Using a multiple recessive marker stock, it was not possible to assign the ant17 to a particular chromosome.