Recombination behavior of repeated sequences in Saccharomyces cerevisiae: Association of intrachromosomal gene conversion and reciprocal exchange.

摘要

There are two types of homologous recombination in eukaryotes: reciprocal exchange (crossing-over) and nonreciprocal exchange (gene conversion). In the fungi, studies of meiotic recombination between single-copy genes located on homologous chromosomes have shown that gene conversion events are associated with crossing-over in flanking regions approximately 50% of the time. Gene conversion has also been shown to occur frequently between repeated genes located on the same chromosome, on homologous chromosomes and on non-homologous chromosomes. However, conversion between repeated genes is rarely associated with reciprocal exchange. To understand the relationship between reciprocal exchange and gene conversion in repeated sequences, the recombination behavior in yeast of an inverted repeat was studied.;Using the kanamycin resistance gene (kan