The levan-ABPC48 idiotype system: Functional and structural aspects.

摘要

The BALB/c myeloma protein ABPC48 (A48) binds bacterial levan (BL) polysaccharide. Its idiotype, designated the A48Id, is expressed by ;Hybridomas secreting A48Id;Another group of hybridomas was obtained from BALB/c mice either untreated or treated at birth with A48 or IDA10. Spleen cells were cultured in vitro with B cell mitogens, fused, and hybridomas selected for expression of V;These mAbs displayed several patterns of binding to polysaccharide-containing antigens. One mAb, and the BALB/c galactan-binding myeloma protein XRPC44, (both expressing V;Idiotopes associated with V