Induction of protective immunity to Mycoplasma gallisepticum by bacterins conjugated with iota carrageenan.

摘要

arious routes of immunization with an inactivated Mycoplasma gallisepticum (MG) bacterin conjugated with 0.2% iota carrageenan (iCGN) were evaluated, to find a regime for immunization which would establish a protective immunity to mycoplasmal challenge. Specific IgG antibodies were measured in sera and tracheobronchial washes (TBW) by an indirect ELISA. Chickens were vaccinated at 3 and 5 weeks of age with the conjugated bacterin administered by various combinations of intracoelomic (IC), intrabursal (IB), intratracheal (IT), intranasal (IN), intravenous (IV), oral and subcutaneous (SC) routes of inoculation. Chickens were challenged by aerosol exposure at 7 weeks of age with an average of 3.0