首页> 外文学位 >Concentration dependency, mode of action and characterization of compounds involved in alfalfa autotoxicity.
【24h】

Concentration dependency, mode of action and characterization of compounds involved in alfalfa autotoxicity.

机译:苜蓿自身毒性中涉及的化合物的浓度依赖性,作用方式和特征。

获取原文
获取原文并翻译 | 示例

摘要

Greenhouse experiments on allelopathy and autotoxicity of 'WL-316' alfalfa (Medicago sativa L.) indicated that plant height and fresh weight of alfalfa, and fresh weight of 'DK-38' sorghum (Sorghum bicolor (L.) Moench) were lower on soil previously cropped to alfalfa, 'alfalfa-soil', than on that previously cropped to sorghum. Allelopathic and/or autotoxic compounds in alfalfa-soil were implicated in the growth inhibition of the two crops. Incorporation of fresh alfalfa residue--roots only or roots and shoots both--reduced alfalfa emergence, plant height and dry weight. Primary effect of water-soluble inhibitory compounds from alfalfa shoot appeared to be on germination and radicle elongation, the latter being more sensitive than the former. Alfalfa allelopathy is a more severe problem than autotoxicity. Bioassay of the alfalfa shoot aqueous extract at 0, 20, 40, 60, and 80 g.L{dollar}sp{lcub}-1{rcub}{dollar} (on a fresh weight basis) under laboratory conditions showed that alfalfa germination, radicle elongation, and shoot elongation were inhibited by the extract in a concentration dependent manner. Shoot extract from reproductive stage was more inhibitory than from vegetative stage. Under greenhouse conditions, alfalfa seedling emergence was adversely affected following incorporation of fresh alfalfa herbage from the vegetative stage at 97 and 146 shoots.m{dollar}sp{lcub}-2{rcub}{dollar} into soil. Among the several phenolic compounds assayed, coumarin and trans-cinnamic acid were highly inhibitory to alfalfa root growth and shoot growth. The mixtures of phenolics were generally more phytotoxic than their respective individual components except in the case of coumarin and trans-cinnamic acid. Scanning electron microscopy techniques, used to study the root morphology and anatomy of the shoot-aqueous-extract-inhibited alfalfa roots showed a 46% reduction in density and 54% reduction in length of root hairs compared to the control. Thin layer chromatography (TLC) of the acid-hydrolysed, ethyl-ether-extracted alfalfa shoot aqueous extract followed by the gas-liquid chromatography of the TLC fractions indicated that some of the compounds present in the sample had characteristics of chalcones and flavones. Medicarpin was not found in the extract.
机译:关于'WL-316'苜蓿(Medicago sativa L.)的化感作用和自身毒性的温室实验表明,苜蓿的株高和鲜重以及'DK-38'高粱(高粱)的鲜重较低。以前种植过苜蓿的土壤,即“苜蓿土”,而不是以前种植过高粱的土壤。苜蓿土壤中的化感和/或自毒化合物与两种作物的生长抑制有关。新鲜苜蓿残留物(仅生根或既有根又有芽)的掺入减少了苜蓿的出苗,株高和干重。苜蓿芽中水溶性抑制化合物的主要作用似乎是对发芽和胚根伸长,后者比前者更敏感。苜蓿化感作用比自体毒性更为严重。在实验室条件下,以0、20、40、60和80 gL {dol} sp {lcub} -1 {rcub} {dol}(以新鲜重量计)的苜蓿芽含水提取物进行生物测定表明,苜蓿发芽,胚根提取物以浓度依赖性的方式抑制了伸长率和枝条伸长率。生殖阶段的芽提取物比营养阶段的芽提取物更具抑制性。在温室条件下,从营养阶段在97和146苗处掺入新鲜的苜蓿草后,苜蓿幼苗的出苗受到了不利影响。m {dollar} sp {lcub} -2 {rcub} {dollar}进入土壤。在测定的几种酚类化合物中,香豆素和反式肉桂酸对苜蓿的根生长和芽生长具有高度的抑制作用。除香豆素和反肉桂酸外,酚类混合物通常比其各自的单独成分更具植物毒性。与对照相比,用于研究抑制水芽提取的紫花苜蓿根的根的形态和解剖学的扫描电子显微镜技术显示,根毛的密度降低了46%,根毛的长度降低了54%。酸水解,乙醚萃取的苜蓿芽水提取物的薄层色谱(TLC),然后TLC馏分的气液色谱分析表明,样品中存在的某些化合物具有查耳酮和黄酮的特征。在提取物中未发现麦草皮。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号