Studies of canine erythrocyte antigens.

摘要

The primary goal of these studies was to develop reagents which would recognize blood group specific antigens on canine erythrocytes. These reagents would be useful in both the clinical and research settings. Methods used in the study included development of monoclonal antibodies, and screening of plant and other sources for naturally occurring substances (lectins), which might recognize canine blood group specific antigens.;A murine IgM monoclonal antibody was produced which recognizes dog erythrocyte antigen (DEA) 1.1. The antibody correctly identified canine red cells possessing DEA 1.1 in a panel of red cells typed by an independent laboratory. Reactivity of the monoclonal antibody was compared to canine anti-DEA 1.1 antiserum with 163 canine red cell samples from 145 different dogs. Results of agglutination tests with the two reagents were in agreement for all samples. A card agglutination test using the monoclonal antibody with whole blood is described.;Agglutination tests were performed with forty purified, commercially available lectins and a panel of blood typed canine erythrocytes (red cell test panel). Lectin reactivity with untreated and enzyme treated cells was determined. No correlation between lectin reactivity and known canine blood groups present on the red cell test panel were found. Results suggest that canine erythrocytes may be differentiated on the basis of reactivity with Phytolacca americana and Glycine max lectins.;Extracts from 117 species of plant seeds were tested for lectin activity against untreated and enzyme treated cells from the red cell test panel. Fifty-three reacted with either untreated or enzyme treated red cells, seven were hemolytic, and 57 were nonreactive. No lectins were found to exhibit blood group specificity. Lectin reactivity for canine red cells is compared to reactivity for human red cells.;Extracts from 69 species of lichens were tested for lectin activity against untreated and enzyme treated cells from the red cell test panel. Forty-three lichen species agglutinated either untreated or enzyme treated red cells. Many extracts exhibited differential agglutination of cells in the red cell test panel, but the patterns of differential agglutination did not correspond to known canine blood groups present on the red cell test panel.