Antigenic differences within Actinobacillus pleuropneumoniae serotype 1.

摘要

Antigenic differences between Actinobacillus pleuropneumoniae subtypes 1A and 1B were analyzed by coagglutination, SDS-PAGE, and immunoblot analysis of outer membranes. The presence of cross-reactive antibodies in sera from pigs immunized with 1A or 1B vaccines was evaluated by ELISA, with 1A or 1B outer membranes as coating antigen. The importance of antigenic differences was determined in a cross-protection study.;Antigenic differences between subtypes 1A and LB were located within the capsular polysaccharides. In immunoblots, sera from vaccinated animals reacted only with the capsular polysaccharides of the homologous strains. Sera from infected animals reacted with all strains regardless of subtype. Differences in immune responses between 1A- and 1B-vaccinated pigs were confirmed by ELISA. Both vaccine types elicited increased antibody levels against homologous and heterologous antigens, but optical density values were usually higher against the homologous antigens. The results of the cross-protection study were inconclusive.