Effects of aging and physical activity on superoxide dismutase in human erythrocytes.

摘要

This study investigated the relationship of the superoxide scavenging enzyme, superoxide dismutase (SOD), with respect to age, gender and physical activity levels of subjects. Blood was collected from 129 healthy human volunteers (80 males and 49 females) between the ages of 7 and 87. A physical activity index (in kcal/wk) was calculated for each subject.;A continuous line assay for Cu,Zn-SOD activity was performed and a function analogous to a Lineweaver-Burke equation in a nonclassical enzyme assay was used to derive a linear reciprocal plot, whose slope was proportional to (kcat Et/Km);With advancing age there was seen to be a gradual decline in (kcat Et/Km);The experimental parameter, (kcat Et/Km)