12(S)-HETE and non-lethal dose of radiation induce vesicle translocation, distribution and trafficking in B16a melanoma cells.

摘要

etastasis is a multistep process involving intravasation, arrest, adhesion to and retraction of endothelium, adhesion to basement membrane and digestion of extracellular matrix, extravasation and finally formation of a secondary tumor. All of these steps must be completed in order to facilitate metastasis. However, adhesion to and dissolution of subendothelial matrix may be the rate-limiting steps.;This study is directed toward understanding the role of the cytoskeleton in organelle translocation, distribution and trafficking in B16a tumor cell line. Stimuli used to initiate cellular responses include exogenous 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE), an arachidonic acid metabolite, and low dose of ;We have characterized the involvement of microtubules and protein kinase C (PKC) in the regulation of organelle translocation of cathepsin L and