Optimization of biocatalytic 3-dehydroshikimic acid production from D-glucose in Escherichia coli.

摘要

iocatalysis is an environmentally friendly alternative to petrochemical processing for production of small molecules. The biocatalytic process objective is efficient and high-yielding conversion of starting material to product. Success of the process depends on manipulation of metabolic fluxes through desired pathways by genetic manipulation and process engineering.;A strain of Escherichia coli was genetically engineered to overproduce dehydroshikimic acid (DHS). This intermediate of the common aromatic amino acid pathway is of commercial interest, because it can readily converted into a variety of valuable products. This E. coli strain features a feedback-insensitive DAHP synthase