De analyse van melphalan en tumor necrose factor-alpha bij chemo-immunotherapie door geisoleerde perfusie.

摘要

During the last few years isolated perfusion becomes more important in the treatment of isolated tumours. New techniques are developed to apply isolated perfusion to the liver and lungs. Bio-analytical methods are necessary to determine concentrations of the administered compounds.; A gas chromatographic-mass spectrometric (GC-MS) method was developed for the determination of L-PAM and its hydrolysis products. TNF-α was determined with a commercial ELISA-kit. However ELISA-tests can produce false positive results by the occurrence of cross reactions of the TNF-α antibody with other proteins. Therefore experiments which can give an impulse to determine TNF-α by capillary electrophoresis (CE) in the future are described in this thesis.; Isolated liver perfusions in pigs with L-PAM, MMC and TNF-α learned us that during an isolated perfusion a 20 to 40-fold concentration advantage for the regional circuit in comparison to the systemic circuit was found.; Isolated lung perfusions with L-PAM are safe and effective for the treatment of lung metastases of adenocarcinoma, in the rat. L-PAM concentrations in the lungs about 50 times higher in the isolated lung perfusion group than in the intravenous group.; Different capillary electrophoretic techniques were evaluated to separate TNF-α from plasma proteins with CE. Qualitative analyses of TNF-α in plasma were performed with this method.