Techniques in microbial and molecular biology: Laboratory techniques for a graduate-level course.

摘要

Advanced laboratory techniques for Microbial and Molecular Biology at the graduate level are presented in this thesis. The procedures for the laboratory experiments are set forth in detail. This laboratory is conducted as two parts, each by a different professor. Part 1 covers the experiments conducted by Dr. G. A. O'Donovan. These experiments include an introduction, staining procedures, biochemical reactions, mutagenesis experiments, assays, preparation and analysis of plasmid DNA and various other topics. Part 2 covers the experiments conducted by Dr. Daniel Kunz and includes various topics like media preparation, phenotyping strains, conjugative transfer of plasmids, SDS-PAGE, induction and measurement of enzyme activity and transposon mutagenesis.