Biocompatibilite, biodegradation et influence de la sterilisation sur le complexe chitosane-xanthane.

摘要

Hydrogels display interesting properties by their network structure allowing a high level water retention without the dissolution of the polymer itself which makes them suitable for controlled drug delivery system. Among them, we studied the chitosan-xanthan (Ch-X) polyelectrolyte complex based on chitosan and xanthan hydrogels. We investigated its biocompatibility, biodegradation and the effects of sterilization on its physico-chemical properties.; The second step of our work consisted on the biodegradation behavior of Ch-X microspheres in simulated media constituted of gastric and intestinal fluids (SGF and SIF). The biodegradation rate was reported by the measurement of glucosamine (GA) and N-Acetyl-glucosamine (N-Ac-GA) peaks area allowing us to quantify the degradation kinetics in the media used.; Based on biocompatibility studies, Ch-X particles, showed no cytotoxic effects, neither their degradation products.; According to in vivo studies, Ch-X hydrogel seemed to be well tolerated by the tissues. The healing process was initiated as shown by collagen deposition, and the fibrous capsule formation did not increase as the degradation of the complex was taking place. In vitro biodegradation studies allowed us to observe that Ch-X provided a protective effect against Ch degradation in both simulated gastric and intestinal fluids. (Abstract shortened by UMI.)