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Studies on the role of DNA looping in lysogenic gene regulation of bacteriophage lambda.

机译:DNA环在噬菌体λ溶源基因调控中的作用研究。

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Gene regulation is sufficiently complex that model systems are very important for advancing the field. Bacteriophage lambda is a classic model for understanding how genes are turned on and off by regulatory proteins. Lambda repressor (or CI) is a phage protein that binds to two well-defined regions of DNA (called operators), serving as an activator as well as a repressor. This thesis examines how long-range interactions between operator-bound CI proteins play a role in regulation of the CI gene. My approach was to use a fluorescent protein to measure how specific changes to the CI-binding properties of the two operators alters the amount of CI produced. From this I discovered that long-range interactions between CI proteins bound at the two operators can increase either activation or repression of the CI gene, depending on the pattern of DNA-binding. I used information from the literature to develop a structural model to represent all the configurations in which CI can bind to unlooped or looped operator DNA. Assigning these configurations as either unactivated, unlooped activated, looped activated or repressed, I calculated the probability of each and solved for the activation level of looped DNA relative to unlooped DNA. I finish this thesis with a discussion of the implications of my results and the additional questions they raise.
机译:基因调控非常复杂,因此模型系统对于推进该领域非常重要。噬菌体λ是一个经典模型,用于了解调节蛋白如何打开和关闭基因。 Lambda阻遏物(或CI)是一种噬菌体蛋白,可与两个明确定义的DNA区域结合(称为操纵子),既可作为激活物,又可作为阻遏物。本文研究了操纵子结合的CI蛋白之间的远程相互作用如何在CI基因的调控中发挥作用。我的方法是使用荧光蛋白来测量两个操纵基因的CI结合特性的特定变化如何改变CI的产生量。由此我发现,结合在两个操纵子上的CI蛋白之间的远距离相互作用可以增加CI基因的激活或抑制,这取决于DNA结合的模式。我用文献中的信息开发了一个结构模型,以表示CI可以结合到无环或环化操纵基因上的所有构型。将这些配置指定为未激活,未激活的环,激活的环或抑制的环,我计算了每种的概率并解决了环DNA相对于未环DNA的激活水平。在结束本文时,我将讨论我的结果的含义以及它们提出的其他问题。

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