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【6h】

Effect of cationic surfactant (N-alkyl trimethylammonium bromide, C(n)TAB) hydrophobicity on cellulase intrinsic fluorescence.

【摘要】 To quantitatively determine the effect of the hydrophobic forces involved in the interaction of cellulase (endo-beta-1,4-glucanase from Aspergillus niger ), and cationic surfactant n-alkyl trimethylammonium bromides (C nTAB), with varying chain lengths (n = 10, 12, 14, 16, 18), steady-state florescence spectroscopy using tryptophan as a probe was employed. No appreciable quenching occurred for the lower chain CnTABs (C10TAB and C12TAB). A linear form of the Stern-Volmer equation modeled the behavior of cellulase quenching by C14TAB, C16TAB, C18TAB, and the KSV values were determined to be 5.9 x 10-5microM-1 (59 M -1), 1.1 x 10-4microM-1 (110 M-1), and 1.4 x 10-4microM -1 (140 M-1), respectively, with R 2 values greater than 0.90 but less than 0.95. The data for the experiments involving C12TAB and C14TAB disagreed significantly with the work of Rastegari et al. in that they differed significantly in the extent of quenching, emission wavelength shift observed from cellulase fluorescence upon addition of the surfactants. None of the results obtained in this experiment were able to confirm the biphasic behavior of endoglucanase. The KSV values obtained from the results are sufficient to claim that higher chain surfactants are more effective quenchers of cellulase fluorescence and consequently, that the hydrophobic forces play a great role in cellulase-CnTAB interactions. A modified form of the equation (having a second order with respect to the concentration of the quencher) distinguishing and modeling both dynamic and static quenching can possibly be applied in the future studies to improve the accuracy of the Stern-Volmer model. Although this would enable to distinguish and perhaps more accurately model the two types of quenching exhibited by the same fluorophore, it would require additional experiments such as measuring fluorescence lifetimes (tau) and/or testing for the effect of temperature.

【作者】 Kim, Doo Won.;

【作者单位】 The Cooper Union for the Advancement of Science and Art.;

【授予单位】The Cooper Union for the Advancement of Science and Art.;

【学科】Chemistry, Analytical.;Engineering, Chemical.;Chemistry, Polymer.

【学位】M.E.

【年(卷),期】2010(),

【年度】2010

【页码】96 p.

【总页数】96

【原文格式】PDF

【正文语种】eng

【中图分类】;

【原文服务方】国家工程技术数字图书馆

【关键词】;

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