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Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375.

【摘要】 For the first time, C60-monoclonal antibody (mAb) immunoconjugates have been determined to internalize into target cells using water-soluble Gd3+-ion-filled fullerenes (Gd C60(OH) x). Separate conjugations of Gd C60(OH)x with the antibody ZME-018 and a murine antibody mixture (MuIgG) took place in a 1:5 mAb:Gd 60 ratio. Quanitative analysis of the immunoconjugates was established using inductively-coupled plasma mass spectrometry (ICP-MS) and UV-Vis spectrometry (Gd C60+C60). Enzyme-linked immunosorbent assays (ELISA) show little change in the specific binding of the ZME-018 once conjugated.;Each immunoconjugate was exposed to two cancer cell lines, A375m (a ZME-018-specific line), and T24, a bladder carcinoma line. Internalization of the immunoconjugate was measured at various timepoints, after which the cells were harvested and digested with 25% HClO3 for Gd3+ analysis by ICP-MS.;ICP-MS results show immunoconjugate internalization peaked in the first hour of exposure of the cells with a large dropoff occuring in the subsequent hour. These are the first results demonstrating the practicality of a cancer therapy based on Fullerene Immunotherapy (FIT).

【作者】 Berger, Christopher Scott.;

【作者单位】 Rice University.;

【授予单位】Rice University.;

【学科】Chemistry, General.;Chemistry, Inorganic.

【学位】M.A.

【年(卷),期】2010(),

【年度】2010

【页码】26 p.

【总页数】26

【原文格式】PDF

【正文语种】eng

【中图分类】;

【原文服务方】国家工程技术数字图书馆

【关键词】;

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