Morphologic and molecular pathogenesis study of condemned kidneys in swine from Alberta.

摘要

Fifty condemned and 10 normal kidneys were collected from slaughtered pigs in southern Alberta and tested for pathogens as etiological agents of interstitial nephritis. A 75% of the samples were positive for bacterial isolation; bacteria caused an unspecific immune response in the host tested by western blot. In 50% of the tissues, porcine parvovirus was detected by PCR; 17.5% of samples were porcine circovirus type 2 positive; and there was no evidence of porcine reproductive and respiratory syndrome virus infection. Fungi and leptospires were not detected by histochemistry and/or immunohistochemistry. The majority of the condemned kidneys (76%) showed subacute to chronic interstitial nephritis; a 16% glomerulonephritis; and 8% had minimal histological changes. In contrast, normal kidneys (70%) were histologically normal. Taken together, these data suggest that the chronicity of the lesions did not determine the etiological agent, and manipulation of these kidneys may not represent a high human health risk.