Mapping and applications of microsatellite and AFLP markers in barley (Hordeum vulgare).

摘要

The objectives of this study were to map both microsatellite and AFLP markers onto segregating barley populations, apply a core set of microsatellite markers to differentiate registered barley lines and to trace the inheritance of chromosomal segments based on pedigree.; A total of twenty new microsatellite markers were added to two existing Harrington x TR306 barley genetic maps. Further sources of microsatellite markers were examined, including wheat microsatellites and probing genomic DNA for repeats, but no additional microsatellite markers were identified.; By using eleven AFLP combinations, 189 polymorphic loci were identified and 138 were mapped onto an existing Shyri x Galena genetic map. The additional markers added 1469 cM to the map and a direct correlation was noticed between the number of AFLP markers added to a chromosome and the increase in chromosome length. Several different mapping experiments were conducted to determine the effects of different classes of markers on the Shyri x Galena map. Mapping of the new AFLP loci increased the overall map length regardless of the marker class excluded from the genetic map.; Two applications of microsatellite markers were applied to barley cultivars. First, a set of nine microsatellite markers, found on five chromosomes, were used to distinguish twenty registered barley cultivars. Secondly, twenty-five microsatellite markers were also screened on 12 barley cultivars and on their parental lines to trace the inheritance of chromosomal segments.