Combinational multiphoton scanning microscopy and multiphoton surgery of mouse arteries.

摘要

Preliminary investigations were carried out in order to explore the potential of laser-stimulated capillary growth in a blood vessel-on-a-chip. To fulfill the project objective, a series of experiments in both directions of two photon fluorescence imaging and laser-semitransparent materials interaction were performed. A purpose-built two-photon fluorescence imaging resolution was tested by imaging 1 micron diameter fluorescent beads. Also, the potential of fluorescence imaging in the waveguide writing field as well as the biological field was studied. Further, for laser ablation on the mouse artery loaded in the microfluidic channel, the processing window was found such that the damage induced by femtosecond laser just a effects the artery, not the other interfaces of the microfluidic chip. At the end, the result of laser trepanning on the mouse artery wall combined with two photon fluorescence imaging was shown. These results will be useful for more advanced biological study such as angiogenesis.