Development of In Vitro Denture Biofilm Models for Studying Denture-related Halitosis and Stomatitis.

摘要

Denture stomatitis and denture-related halitosis are two of the most prevalent denture related infectious diseases. The establishment of denture biofilm models for disease-associated pathogens is essential in further investigating the pathogenesis of these diseases. Chapter I and II of this thesis reported the successful development of denture biofilm model for Candida albican, the main pathogen of denterun stomatitis; as well as the denture models for halitosis-related bacteria, including Klebsiella pneumonia, Fusobacterium nucleatum, Tannerella forsythia, Veillonella atypica. We further demonstrated that the established denture models can be used for evaluating efficacy of denture-cleansers. Chapter III is focused on further exploring the association between denture stomatitis and denture-related halitosis. We revealed the interaction between C. albican and halitosis related pathogens using in vitro co-aggregation assay. A further investigation of the possible association between stomatitis-associated C. albicans and halitosis-associated bacteria in vivo is necessary.