Proteome profiling of human heart tissues and squamous carcinoma cells.

摘要

This work focuses on two important areas of proteomics research: comprehensive proteome profiling of heart tissues, and relative quantification of proteins from squamous carcinoma cell lines and heart tissues. Trypsin digestion and microwave-assisted acid hydrolysis (MAAH) were used to generate peptide mixtures that were fractionated by two-dimensional liquid chromatography (LC) and analyzed by electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) tandem mass spectrometry (MS). The proteome information generated by these methods can be used for understanding the protein expression alterations in related cell lines as well as failing heart tissues and other disease states.