Dietary soy protein and renal disease selectively alter cyclooxygenase isoforms and prostanoid production in Han:SPRD-cy rats.

摘要

Dietary soy protein feeding initiated at weaning slows the progression of renal injury and inflammation in the Han:SPRD-cy rat model of autosomal dominant polycystic kidney disease (ADPKD). However, the effects of initiating dietary soy protein prior to weaning have not been studied. Cyclooxygenase (COX) isoforms are altered in the kidneys of these rats. Therefore, the objectives of this study were two fold: (1) to determine the effects of initiating dietary soy protein prior to weaning, and (2) to examine the effects of disease and dietary soy protein on renal prostanoid production and COX isoforms. Han:SPRD-cy rats were given casein or soy protein in the maternal and/or post-weaning diets in a 2x2 design. Dietary soy protein in the post-weaning diet reduced cyst growth, inflammation and cell proliferation, as previously reported. Dietary soy protein in the maternal diet also independently reduced renal inflammation by 22% and lowered proteinuria by 33%. Soy protein in either the maternal or post-weaning diet reduced renal cell proliferation, with either intervention being equally effective. Soy protein in the post-weaning diet resulted in lower levels of particulate cPLA2, but did not affect COX isoform levels; soy protein in the maternal diet did not alter the levels of cPLA2 or COX isoforms. Therefore, the effects of disease and soy protein on prostanoids and COX isoform activities were examined in rats given soy in the post-weaning diet. The presence of disease resulted in higher kidney levels of TXA2, PGE2 , PGI2 and COX isoform activities. (Abstract shortened by UMI.)