Multi-camera Laser Speckle Imaging System Implementation.

摘要

Laser speckle contrast imaging (LSI) is a noninvasive technique that can rapidly characterize blood perfusion in the microvasculature. For skin perfusion study, researchers usually study on subjects with a small field of views (e.g. palm, cheek) due to limited laser irradiance, curvilinear surface and Nyquist sampling requirements. For human skin study that requires a larger field of view, such as the burn wound assessment, we propose an improved imaging method, multi-camera laser speckle imaging (mcLSI), consisting of multiple LSI modules and including concepts of image stitching. We conduct in-vitro experiments on a flat and a cylindrical phantom using a conventional LSI and an mcLSI device. In-vivo demonstration results of post-occlusion reactive hyperemia test were also shown in the thesis. Our results show that with mcLSI, we are able to acquire images with a larger field of view, a larger range of speckle contrast, and a higher spatial resolution compared to conventional LSI. Furthermore, it also well manages the curvilinear surface area of the body by allowing each LSI module to be set at different angles.