Function and regulation of the clasp-dependent microtubule array at the Golgi.

摘要

The work presented in this thesis follows up on our initial finding that the Golgi apparatus serves as an additional MTOC within the cell (Efimov et al., 2007). Not only did this publication identify a new subset of cellular MTs; it also opened the door to many unanswered questions: What is the functional significance of Golgi-derived MTs? How is the CLASP-dependent MT array at the Golgi regulated?;In Chapter II, I highlight my efforts that contributed to the publication in Developmental Cell in 2007 (Efimov et al., 2007) that initially identified the Golgi apparatus as an additional MTOC. Chapters III and IV focus more on the function of the Golgi-derived MT array. Chapter III discusses how Golgi-derived micotubules contribute to the establishment of an asymmetric MT array that is required for directional cell migration and Chapter IV highlights the importance of this microtubule subset in Golgi assembly and organization, polarized post-Golgi trafficking, and directional cell migration. Switching gears, Chapter V focuses on the regulation of the Golgi-derived MT array via GSK3beta regulation of Golgi-associated CLASPs. Overall, this thesis contributes to a better understanding of the functional significance and regulation of the CLASP-dependent MT array at the Golgi.