Detection of prostate cancer-related gene fusions using nanowire electrodes.

摘要

DNA detection is an important analytical tool in disease diagnosis and treatment. Among all the DNA detection methods, electrochemical DNA sensing methods are the most promising in clinical use because quantitative measurements can be made and large sample processing is unnecessary. Recently, the combination of nanotechnology and electrochemical sensing methods has introduced great sensitivity and specificity in DNA detection, providing powerful devices that can be used in large-scale genetic testing.; This thesis focuses on an electrochemical assay for the detection of prostate cancer related gene fusions. Using an electrocatalysis coupled nanowire electrode platform, we have developed a sensitive assay for the detection of the TMPRSS2:ERG fusion gene sequence, which may serve as a specific biomarker in prostate cancer diagnosis.