Biophysical applications of single molecule fluorescence localization.

摘要

Fluorescence single molecule localization has become a powerful tool for investigating problems in biological physics. However, there are numerous limitations on the types of measurements that can be made with typical instrument designs based on objective-type TIR and wide field CCD imaging. We present several novel applications of single molecule localization to both proteomics and genomics, including a method for improving the resolving capacity by more than an order of magnitude (Single Molecule High Resolution Imaging with Photobleaching, or SHRImP), a method for extending wide field single-molecule localization to three dimensions (Three Dimensional Imaging with One Nanometer Accuracy, or 3IONA), and applications of these techniques to problems in genomics and molecular motors.