Mouse brain atlasing.

摘要

Data collected from mouse models of human disease have produced an enormous glut of information that requires new methods to interact with and analyze it. We developed digital atlases for the organization, integration, and analysis of mouse brain data. A reference atlas was constructed using magnetic resonance microscopy (MRM), blockface imaging, histology, and immunohistochemistry. Two dimensional histology and immunohistochemistry stained sections were reconstructed into three dimensional image volumes. These volumes were in turn registered into a common atlas space. Visualization tools were developed interaction and analysis of the data. A probabilistic atlas was then constructed and a deformable atlas technique was used to measure structural changes in the brains of mice with the mouse model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE).