The effects of temperature and admixture handling on lipid emulsion stability in centrally administered veterinary parenteral nutrition (PN) admixtures.

摘要

Objective. To determine the temporal change in lipid particle size in veterinary parenteral nutrition (PN) admixtures kept at room temperature (23°C) versus admixtures filtered, refrigerated, and agitated.;Procedure. Fifteen 2 L bags of PN admixture containing 50% dextrose (525 mL), 20% lipid emulsion (453 mL), 8.5% amino acids/electrolyte solution (840 mL) and vitamin B complex (5 mL) were delivered through an intravenous pump (16 mL/hr) for 96 hours. Group 1 (n=3) was static, Group 2 (n=3) was continuously agitated, Group 3 (n=3) was agitated for 5 minutes every 4 hours, Group 4 (n=3) was static at 4°C, and Group 5 (n=3) was filtered (5 mum pore). After 96 hours, two 10 mL samples of PN (n=3) were cultured (bacterial). Samples (1.0 mL) were collected at 0, 24, 48, 72, and 96 hours and examined with transmission electron microscopy. Computer software (Adobe Photoshop & Fovea Pro) provided lipid particle diameters. Significance of time effects on size distribution was evaluated with Repeated Measures ANOVA.;Results. There was no significant difference in lipid particulate size among or within groups over time (p≤0.05). Group 2 separated into a visible oil layer by 72 hours. There was no bacterial growth (aerobic or anaerobic).;Conclusion. Lipid particulate size is stable in this veterinary PN admixture for more than 48 hours at 23°C. Manipulations of PN are unnecessary to prolong lipid particle stability; continuous agitation may hasten lipid breakdown.