Biomarkers of systemic disease in dogs and cats.

摘要

Microalbuminuria assays were compared with clinical diagnoses recorded within 3 months of urinalyses in dogs without overt proteinuria and in cats. A Western blot immunoassay was developed for detection of antibodies to Crandell Rees Feline Kidney (CRFK) cell proteins in sera. Immunodominant CRFK proteins were identified. Antibody development patterns were determined for cats inoculated with CRFK proteins or FVRCP vaccines. The Western blot immunoassay was adapted to detect CRFK antigens in feline and murine tissues. Associations between CRFK antibodies and vaccination were determined. Associations between CRFK antibodies and biochemical abnormalities were determined in 1,477 privately-owned cats.; Dogs positive by quantitative and semiquantitative microalbuminuria assays were more likely to have systemic disease (2.3 and 4.8 times) and neoplasia (2.7 and 8.1 times). Positive microalbuminuria results were also significantly associated with age, BUN, creatinine, and hematuria. Cats positive by quantitative and semiquantitative microalbuminuria assays were more likely to have systemic disease (6.7 and 2.4 times). Positive microalbuminuria results were significantly associated with BUN, creatinine, age, pyuria, and hematuria. Gender was not significantly associated with microalbuminuria. The urine albumin:creatinine ratio had poor sensitivity for systemic disease in both species.; Crandell Rees Feline Kidney cell protein inoculation and FVRCP vaccination were associated with autoantibody development measurable by Western blot immunoassay. Antibody development occurred as early as four weeks after vaccination. The number and density of antibody bands was greater after one year booster inoculation. Antibody bands developed against antigens 47kD, 40kD and 38kD in size. Immunodominant CRFK antigens were identified as alpha-enolase, annexin A2, and macrophage capping protein. Wide tissue distributions for alpha-enolase, annexin A2, and macrophage capping protein were found in cats, consistent with the human literature. Significant associations were found between anti-CRFK antibody levels and biochemical abnormalities. There was a significant positive correlation between anti-CRFK antibody levels and serum bilirubin levels. There were significant negative associations between anti-CRFK antibody levels and serum creatinine, ALP activity, and glucose.