首页> 外文学位 >Genetic diversity of Rhizoctonia solani causing web blight and sources of resistance in Phaseolus spp.
【24h】

Genetic diversity of Rhizoctonia solani causing web blight and sources of resistance in Phaseolus spp.

机译:造成网叶枯萎病的茄枯萎菌的遗传多样性和菜豆的抗性来源。

获取原文
获取原文并翻译 | 示例

摘要

Web blight of common beans caused by Rhizoctonia solani is a very important disease in bean growing regions of Latin America and the Caribbean. There are no cultivars with high levels of resistance available mostly because of a lack of information on the pathogen genetic diversity and virulence patterns and the need to find sources of resistance that can be introgressed into common bean.;To study virulence patterns, the development of a set of differential bean lines is essential. Two groups of candidate bean lines were screened with isolates from AG 1-1F, AG 1-1E and AG 1-1A using the detached leaf technique. No significant differences were found among lines at any of the different growing conditions tested. All lines were rated as susceptible to all isolates even though they were selected based on the moderate resistance they showed in field trials. Thus new screening methods and more cultivars/lines need to be studied in order to develop a set of differentials.;Genetic diversity of isolates collected in Puerto Rico, Honduras and the Dominican Republic over ten years from the two newly described subgroups AG 1-1E and AG 1-1F was assayed with four molecular markers. While some isolates were found to be clonal, Universal Rice Primers (URP) markers were the most informative showing differences between countries and years of collection for most of the isolates. This would support the hypothesis that population structure has changed over time and that there could be sexual recombination among a few genotypes.;A group of 71 accessions from several species of Phaseolus including wild and landrace collections was tested for resistance to isolates from AG 1-1F, AG 1-1E and AG 1-1F. Fifteen lines were classified as moderately resistant to AG 1-1A but susceptible to the other two subgroups. Line 01-006-3B from P. coccineus was found to be resistant to AG 1-1F, intermediate to AG 1-1E and susceptible to AG 1-1A making it the best line to be considered for common bean breeding programs wanting to introgress web blight resistance into commercial varieties.
机译:solani引起的普通豆网枯病在拉丁美洲和加勒比海的豆类生长地区是一种非常重要的疾病。没有可用的高水平抗性品种,主要是因为缺乏有关病原体遗传多样性和毒力模式的信息,以及需要寻找可以渗入普通豆的抗性来源的研究;一组不同的Bean线至关重要。使用离体叶片技术,用来自AG 1-1F,AG 1-1E和AG 1-1A的分离株筛选两组候选豆系。在测试的任何不同生长条件下,品系之间均未发现显着差异。即使根据在田间试验中显示的中等抗性选择了所有品系,也将所有品系定为对所有分离株敏感。因此,需要研究新的筛选方法和更多的品种/品系,以建立一组差异。十年来,从波多黎各,洪都拉斯和多米尼加共和国两个新描述的亚组AG 1-1E收集的分离株的遗传多样性AG 1-1F用四种分子标记进行测定。尽管发现了一些分离株是克隆的,但通用水稻引物(URP)标记是提供最多信息的标记,显示大多数分离株的国家和收集年限之间存在差异。这将支持以下假设,即种群结构随时间变化并且少数基因型之间可能发生性重组。;测试了来自菜豆(Phaseolus)几种物种(包括野生和地方品种)的71种保藏物对AG 1分离株的抗性1F,AG 1-1E和AG 1-1F。十五个品系被分类为对AG 1-1A具有中等抵抗力,但对其他两个亚组敏感。发现来自球孢假单胞菌的品系01-006-3B对AG 1-1F有抗性,在AG 1-1E中间,并且对AG 1-1A敏感,使其成为希望进行渗入的普通豆育种计划的最佳品系卷材抗白叶枯病成为商业品种。

著录项

  • 作者单位

    The University of Nebraska - Lincoln.;

  • 授予单位 The University of Nebraska - Lincoln.;
  • 学科 Biology Microbiology.;Agriculture Plant Pathology.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 231 p.
  • 总页数 231
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;植物病理学;
  • 关键词

  • 入库时间 2022-08-17 11:39:00

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号