声明
论文说明
摘要
Abstract
1 Introduction
1．1Bacteria resistance against antibiotics
1．2．1CysM Protein
1．2．2Role of Cysteine
1．3Bacteria resistance prevention and control
1．4Chinese Herbs Medicine against bacteria resistance of S．suis
1．5Gallic acid
1．5．1Introduction to Gallic acid
1．5．2Preventive and therapeutic uses of Gallic acid
1．6Purpose and significance of the study
2 Materials and Methods
2．1Materials
2．1．1Softwares and Websites
2．1．2Test strains
2．1．3Drugs
2．1．4Instrument and equipment
2．1．5Traditional Chinese Madieines ingredients
2．2Methodology
2．2．1Homology modeling
2．2．2Model Optimization and Validation
2．2．3Ligands preparations
2．2．4CysM Protein preparation
2．2．5Molecular Docking of CysM
2．2．6Study of traditional Chinese Medicine properties
2．2．7Antibacterial effect of TCMs on Streptococcus suis
2．2．8MIC of TCMs on Streptococcus suis induced tylosin
2．2．9The MIC of TCMs on Streptococcus suis induced tylosin with cysM gene knockout
2．2．10Statistical analysis
3 Results and analysis
3．1．2PROCHECK Evaluation of CysM
3．1．3Errat Evaluation
3．1．4 Verify 3D Evaluation
3．2 Predict the combination of Gallic Acid and CysM
3．3ADMET results prediction
3．4Evaluation of antibacterial effect of TCMs on Streptococcus suis wild type strain，tylosin antibiotic and cysM mutant in vitro
3．4．1Wild type strain
3．4．2Tylosin antibiotic
3．4．3cysM mutant
4 Discussion
4．1Homology model and Sequence alignment of CysM
4．2Study of Traditional Chinese Medicine
4．2．1Docking Result Analysis
4．2．2ADMET Result Analysis
4．3Antibacterial effect of Traditional Chinese Medicine on Streptococcus suis
4．4Inhibitory effect of Traditional Chinese Medicines on Streptococcus suis with cysM mutant
5 Conclusion
Acknowledgement
References
Papers published in the period of Ph．M．education
东北农业大学;
