声明
Contents
Abstract
摘要
List of Abbreviation
CHAPTER 1 Literature review
1．1 Introduction to the oriental armyworm，Mythimna separata
1．1．1 Biological group and status
1．1．2 Morphology
1．1．3 Life history
1．1．4 Distribution and damage
1．1．5 Pest management
1．2 Insect Phototaxis
1．2．1 Understanding on insect phototaxis
1．2．2 Hypotheses on phototactic behavior of insect to light
1．2．3 Factors affecting insect phototactic behavior
1．2．4 Phototactic behavioral responses of insects to different light wavelengths
1．3 Influence of light illumination on biological response of insect
1．3．1 Effect on biochemical responses
1．3．2 Effect on proteomic and molecular responses of insect
1．3．3 Effect on biological characteristics of insect
1．3．4 Effect on juvenile hormone
1．4 Considerable problems in the application of light traps
1．5 Objectives and significance of this study
1．6 Technical route of the study
CHAPTER 2 Phototactic response of the oriental armyworm，Mythimna separata，to LED lights of different wavelengths
2．1 Introduction
2．2 Materials and methods
2．2．1 Experimental insects
2．2．2 Y-maze bioassay chamber
2．2．3 Light sources and luminante intensity measurement tool
2．2．4 Experiment in the laboratory
2．2．5 Experiment in the net setup
2．2．6 Statistical analysis
2．3 Results
2．3．1 Phototactic response of M．separata moths to LED lights of different wavelengths under several light luminance intensities
2．3．2 Phototactic response of M．separata moths under the optimum conditions of each LED light
2．3．3 Phototactic response of M．separata moths to LED lights of several wavelengths and combined wavelengths
2．3．4 Phototactic response of M．separata moths under the net setup condition
2．4 Discussion
CHAPTER 3 Influence of several factors on the phototactic response of the oriental armyworm，Mythimna separata，to green LED light
3．1 Introduction
3．2 Materials and methods
3．2．1 Experimental insects
3．2．2 Light sources and intensity measuring tool
3．2．3 Phototactic response test chamber
3．2．4 Bioassay
3．2．5 Statistical analysis
3．3 Results
3．3．1 Effect of light exposure time
3．3．2 Effect of light luminance intensity
3．3．3 Effect of dark adaptation time
3．3．4 Effect of adult age
3．3．5 Effect of mating status
3．3．6 Effect of adult sex
3．4 Discussion
CHAPTER 4 Influence of green LED light on several enzymes involved in energy metabolism in the oriental armyworm，Mythimna separata
4．1 Introduction
4．2 Materials and methods
4．2．1 Experimental insects
4．2．2 Treatment of green LED light illumination
4．2．3 Sample preparation
4．2．4 Assay of enzyme activities
4．2．5 Statistical analysis
4．3 Results
4．3．1 Influence on GAPDH activity
4．3．2 Influence on GPDH activity
4．3．3 Influence on LDH activity
4．3．4 Influence on HAOD activity
4．3．5 Influence on ratio of HOAD to GAPDH activity
4．4 Discussion
CHAPTER 5 Influence of green LED light on antioxidant enzymes in the oriental armyworm，Mythimna separata
5．1 Introduction
5．2 Materials and methods
5．2．1 Experimental insects
5．2．2 Treatment of green LED light irradiation
5．2．3 Sample preparation and measuring a protein concentration
5．2．4 Assay of total antioxidant capacity and antioxidant enzymes
5．2．5 Statistical analysis
5．3 Results
5．3．1 Influence on T-AOC
5．3．2 Influence on SOD activity
5．3．3 Influence on CAT activity
5．3．4 Influence on POD activity
5．3．5 Influence on GST activity
5．4 Discussion
CHAPTER 6 Influence of green LED light on juvenile hormone in the oriental armyworm，Mythimna separata
6．1 Introduction
6．2 Materials and methods
6．2．1 Experimental insects
6．2．2 Green light treatment
6．2．3 Sample preparation and JH assay
6．2．4 Statistical analysis
6．3 Results
6．3．3 JH titer of adults
6．4 Discussion
CHAPTER 7 Influence of green LED light on biological characteristics of the oriental armyworm，Mythimna separata
7．1 Introduction
7．2 Materials and methods
7．2．1 Experimental insects
7．2．2 Experimental groups of P generation and F1 generation
7．2．3 Light treatment
7．2．4 Adult longevity and reproduction
7．2．5 Classification of experiments
7．2．6 Statistical analysis
7．3 Results
7．3．1 Experiment 1
7．3．2 Experiment 2
7．3．3 Experiment 3
7．3．4 Experiment 4
7．4 Discussion
CHAPTER 8 Conclusions and prospects
8．1 Conclusions in this study
8．2 Scientific innovations
8．3 Prospect of this study in the future
References
Appendix
Acknowledgements
