Characterization of the Complete Mitochondrial Genomes of Nematodes Ostertagia Trifurcata and Trichuris Skrjabini and Their Phylogenetic Implications

目录

声明

论文说明

TABLE OF CONTENTS

摘要

ABSTRACT

CHAPTER 1 REVIEW OF LITERATURE

1．1 Background

1．2 Progressive goat industry in China

1．3 Parasitology

1．3．1 Ostertagia spp．

1．3．2 Trichuris spp．

1．4 Overview of mitochondria and mt genome sequencing

1．4．1 Mitochondria

1．4．2 Evolution of mitochondria

1．4．3 Mitochondrial Gene Content of Animals

1．4．4 Mitochondrial Genome Structure

1．5 Molecular Biology

1．5．1 Phyiogenetic studies

1．6 Next generation sequencing (NGS) technology and parasite genomlcs

1．7 Parasite Genomes

1．7．1 Mitochondrial genomes of parasites

1．7．2 Workflow based on NGS for mitochondrial genome

Objectives

CHAPTER 2 CHARACTERIZATION OF THE COMPLETE MITOCHONDIUAL GENOME OF OSTERTAGIA TRIFURCATA OF SMALL RUMINANTS AND ITS PHYLOGENETIC ASSOCIATIONS FOR THE TRICHOSTRONGYLOIDEA SUPERFAMILY

1．Introduction

2．Materials and Methods

2．1 Collection of worms and extraetion of DNA

2．2 Amplification of ITS-2 of Ostertagia trifurcata

2．3 Amplification of long fragments and sequencing

2．4 Gene annotation and sequence analysis

2．5 Phylogenetic analysis on basis of dataset of amino acid sequences

3．Results and Discussion

3．2 Organization，content and mt genome annotation

3．3 Phylogenetic analysis

4．Implications and Significance

5．Conclusion

CHAPTER 3 CHARACTERIZATION OF THE COMPLETE MITOCHONDRIAL GENOME OF A WHIPWORM TRICHURIS SKRJABINI (NEMATODA：TRICHURIDAE)

1．Introduction

2．Materials and Methods

2．2 Trichuris skrjabini ITS-2 Amplification

2．3 Amplification of short and long fragments by PCR

2．4 Gene annotation and sequence analysis

2．5 Phylogenetic analysis

3．Results

3．1 ITS-2 analysis

3．2 Amplification of shortand long fragments

3．3 mt Genome Features

3．4 Annotation

3．5 Phylogenetic analysis

4．Discussion

5．Conclusion

CHAPTER 4 SUMMARY

REFERENCES

APPENDIX

ACKNOWLEDGEMENTS

CURRICULUM VITAE