Direct writing of a protein microarray: lab-on-a-chip for multipurpose sensing

摘要

A protein microarray has been realized on a porous silicon (PS) chip by means of electron beam irradiation using a standard SEM equipped with a nanopattern generator system. Two proteins have been used to generate the array: the glucose-binding protein (GBP) and the glutamine-binding protein (GlnBP), both isolated from Escherichia coli. The proteins functionality have been tested by means of a competitive assay.