首页> 外文会议>Thermal Treatment of Tissue: Energy Delivery and Assessment III; Progress in Biomedical Optics and Imaging; vol.6, no.13 >Effects of thermal therapy on intervertebral discs: investigations using a miniaturized RF heating probe in a small animal model
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Effects of thermal therapy on intervertebral discs: investigations using a miniaturized RF heating probe in a small animal model

机译:热疗法对椎间盘的影响:在小型动物模型中使用微型射频加热探针进行的研究

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Several studies have suggested that thermal therapy has a beneficial effect on degenerated intervertebral discs. Possible therapeutic mechanisms include collagen denaturation, cell ablation, and nerve tissue coagulation, however the precise mechanism or combination of mechanisms is unknown. To investigate the in vivo effects of thermal therapy on intervertebral discs using a murine model, a monopolar RF heating probe was developed and characterized. In addition, the effect size of several different thermal exposures was investigated by quantifying regions of cell death and collagen denaturation in rat discs. The heating probes were fabricated to have a 0.3mm outer diameter, 0.9mm exposed heating tip, and contain a thermocouple to monitor probe temperature. Using less than 2 Watts of RF energy produced high temperatures close to the probe and a steep radial temperature falloff. Five discs were treated at each of seven thermal exposures: 80℃ for 10min, 80℃ for 1 min, 75℃ for 5 min, 62℃ for 10min, 55℃ for 15min, 48℃ for 15min, and 48℃ for 5min. A cell death region was observed in all treated discs and was significantly greater at the 80℃ for 10min exposure than any of the lower exposures. Denaturation area was significantly greater at the 80℃ for 10min exposure than at the four lowest temperature exposures. Denaturation was not observed in the 48℃ exposures and was inconsistent in the 55℃ and 62℃ exposures. These results demonstrate that it is possible to limit and target the effects of thermal therapy to a portion of the murine disc using this RF probe.
机译:几项研究表明,热疗法对变性椎间盘有有益作用。可能的治疗机制包括胶原蛋白变性,细胞消融和神经组织凝结,但是确切的机制或机制的组合尚不清楚。为了研究使用鼠模型对椎间盘进行热疗的体内效果,开发并表征了单极射频加热探针。此外,通过量化大鼠椎间盘中细胞死亡和胶原变性的区域,研究了几种不同热暴露的效应量。加热探针的外径为0.3毫米,暴露的加热尖端为0.9毫米,并装有热电偶以监测探针温度。使用少于2瓦的RF能量会产生接近探头的高温,并且径向温度急剧下降。在七个热暴露中分别处理五个圆盘:80℃10分钟,80℃1分钟,75℃5分钟,62℃10分钟,55℃15分钟,48℃15分钟和48℃5分钟。在所有处理过的椎间盘中均观察到细胞死亡区域,并且在80℃暴露10min时其细胞死亡区域明显大于任何较低暴露区域。在80℃下暴露10min的变性面积明显大于在四个最低温度下暴露的变性面积。在48℃的暴露条件下未观察到变性,而在55℃和62℃的暴露条件下则未发现变性。这些结果表明,使用该RF探针可以将热疗法的作用限制和靶向于鼠盘的一部分。

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