The Effects of Matrix Metalloproteinase-9 on Dairy Goat Mastitis and Cell Survival of Goat Mammary Epithelial Cells

摘要

Matrix metalloproteinase-9 (MMP-9) is a zinc-dependent enzyme, and plays a crucial role inrnextracellular matrix degeneration, inflammation and tissue remodeling. However, thernrelationship between MMP-9 and somatic cell count (SCC) in goat milk and the role ofrnMMP-9 in the regulation of mastitis are still unknown. In this study, we found MMP-9 wasrnpredominantly expressed in the spleen, intestine and mammary gland. The SCC in goat milkrnwas positively correlated with MMP-9 expression, and staphylococcus aureus could markedlyrnincrease MMP-9 expression in goat mammary epithelial cells (GMEC) in dosage and timerndependent manner. We also demonstrated that SB-3CT, an inhibitor of MMP-9, promotedrnapoptosis and inhibited proliferation in GMEC. Thus, MMP-9 may be a potential diagnosticrnmarker for goat mastitis and a regulatory factor of apoptosis in GMEC.