Screening and Phylogenetic Analysis of Halophilic Bacteria from the Deep Sea Water at Low Temperature

摘要

Three strains of halophilic bacteria were screened from the deep sea water at low temperature. Their phylogenetic positions on the basis of amplification, comparison and analysis of almost complete 16S rRNA gene were cloned and determined by neighboring-joining analyses. Phylogenetic analysis indicated that two strains belong to Pseudomonas.sp. The third stain was classified as genus Acinetobacter.sp. The phylogenetic tree was produced from 16S rRNA of 3 halophilic strains and other 30 strains using 16S rRNA gene as out-group. Two strains (B2 and CY11) were clustered with many Pseudomonas.sp strains. One strain (LT4) was clustered with Acinetobacter baumannii.